Huma Aslam Bhatti
University of Karachi
Title: Identification of New Potent Inhibitor of Aldose Reducatse from Ocimun Basilicum
Biography
Biography: Huma Aslam Bhatti
Abstract
Recent efforts is to develop cure for chronic diabetic complications results in the discovery of potent inhibitors against aldose reductase (ALR2, EC 1.1.1.21) whose role in diabetes is well-evident. In the present work, two new natural products were isolated from the ariel part of Ocimumbasilicum;7-(3-hydroxypropyl)-3-methyl-8-β-O-D-glucoside-2H-chromen-2-one (1) and E-4-(6'-hydroxyhex-3'-en-1-yl)phenyl propionate (2) and confirmed their structures with different spectroscopic techniques including NMR spectroscopyetc. The isolated compounds (1, 2) were evaluated for in vitro inhibitory activity against aldose reductase (ALR2) and aldehyde reductase (ALR1).The natural product (1) showed better inhibitory activity for ALR2 with IC50 value of 2.095 ± 0.77 µM compare to standardsorbinil (IC50 = 3.14 ± 0.02µM). Moreover, the compound (1) also showed multifoldshigher activity (IC50 = 0.783 ± 0.07µM) against ALR1 as compared to standard valproic acid (IC50 = 57.4 ± 0.89µM). However, the natural product (2) showed slightly lower activity for ALR2 (IC50 = 4.324 ± 1.25µM). Moreover, the molecular docking studies of the potent inhibitors were also performed to identify the putative binding modes within the active site of aldose/aldehyde reductases.